Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Satb2

Cell type

Cell type Class
Neural
Cell type
Cerebellar Cortex
MeSH Description
The superficial GRAY MATTER of the CEREBELLUM. It consists of two main layers, the stratum moleculare and the stratum granulosum.

Attributes by original data submitter

Sample

source_name
cortex
tissue
cortex
strain
CD1
age
embryonic day 15
chip antibody
SATB2 antibody

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate from sonicaed cortices were cleared after centrifugation. Satb2-DNA complexes were isolated using a Satb2 antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). ChIP-Seq library was sequenced for pair-end sequencing (100 nt per end) on an Illumina Genome Analyzer II platform

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
35697107
Reads aligned (%)
85.2
Duplicates removed (%)
10.3
Number of peaks
487 (qval < 1E-05)

mm9

Number of total reads
35697107
Reads aligned (%)
85.1
Duplicates removed (%)
10.3
Number of peaks
456 (qval < 1E-05)

Base call quality data from DBCLS SRA