Antigen

Antigen Class

TFs and others

Antigen

Satb2

Cell type

Cell type Class

Neural

Cell type

Cerebellar Cortex

MeSH Description

The superficial GRAY MATTER of the CEREBELLUM. It consists of two main layers, the stratum moleculare and the stratum granulosum.

Sample

source_name

cortex

tissue

cortex

strain

CD1

age

embryonic day 15

chip antibody

SATB2 antibody

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysate from sonicaed cortices were cleared after centrifugation. Satb2-DNA complexes were isolated using a Satb2 antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). ChIP-Seq library was sequenced for pair-end sequencing (100 nt per end) on an Illumina Genome Analyzer II platform

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

35697107

Reads aligned (%)

85.2

Duplicates removed (%)

10.3

Number of peaks

487 (qval < 1E-05)

mm9

Number of total reads

35697107

Reads aligned (%)

85.1

Duplicates removed (%)

10.3

Number of peaks

456 (qval < 1E-05)